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Ready-to-use culture media or concentrated reagent kits (BG11, M7, S2T2, F/2 with different salinities, LC, Z8, MHY) Soil and Sphagnum extract for M7, S2T2, and LC media. The composition of the culture medium is sent with the medium.

Public deposit of microalgae and cyanobacteria

Extraction, hydrolysis, identification, and quantification of monosaccharides from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-RI).

Extraction, identification and quantification of pigments from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-DAD)

Extraction, identification and quantification of fatty acids from microalgae and cyanobacteria by gas chromatography (GC-FID).

Extraction and quantification of total lipid from microalgae and cyanobacteria by gravimetric methods; separation and quantification of neutral lipids, glycolipids and phospholipids by column chromatography

Extraction and quantification of total sugars of microalgae and cyanobacteria by gravimetric analysis and spectrophotometry

Genomic DNA from microalgae and cyanobacteria from collection, minimum of 2 µg of DNA. Qualitative and quantitative assessment by spectrophotometry.

Isolation and preservation of microalgae and cyanobacteria (routine subculture and cryopreservation)

Microalgae and cyanobacteria live strains

Taxonomic identification of microalgae and cyanobacteria using a polyphasic approach (microscopy, molecular biology, biochemistry).

The Karl Fischer titration is a sensitive method to quantify the % water in various types of samples.

Mass percentage (% m/m) quantification of carbon (C), hydrogen (H), nitrogen (N), sulfur (S) and oxygen (O) present in a sample.

Quantitative and qualitative determination of ions (anions and cations).

Separation of the various components of a mixture, with the aim of identifying and quantifying these components.

Spectroscopic analysis methods consist of measuring the amount of radiation emitted or absorbed by molecules.

Study of the deformational behavior and the flow of matter subjected to stress, under certain thermodynamic conditions over a period of time. It includes properties such as: elasticity, viscosity and plasticity.

Determination of particle sizes (from 3 nm to 5000 μm). Aggregation, agglomeration and flocculation studies.

Determination of surface chemical and physical properties.

Study of the behavior of materials as a function of time or temperature, when heated, cooled or kept at a constant temperature.

Investigations on chemical reactivity at low-temperatures (down to 4 K) trigger by narrowband UV-Vis-IR radiation and using steady-state FTIR spectroscopy

i) fluorescência em estado estacionário ii) fluorescência resolvida no tempo

Singlet oxygen detection and quantification.

i) viabilidade celular; ii) fototoxicidade; iii) "uptake"; e iv) mecanismo de morte celular

Infrared and Raman spectroscopic analysis applied to materials and food science, astrochemistry, arts and cultural heritage, and fundamental science

Multivariate statistical analysis and extracting information from spectral data through chemometrics

High precision thermochemistry studies of transient species. Quantum yields of fast photoinduced processes. Detection of fast structural volume changes in molecules and macromolecules.

Based on acoustic detection of optical absorption from endogenous chromophores (e.g. hemoglobin) or exogenous contrast agents (organic dyes or nanoparticles), it is an imaging modality with great potential for preclinical research and clinical practice.

i) estudos de fotoestabilidade incluindo avalição de fotocatalisadores, etc; ii) estudos espectroscópicos UV-Vis-NIR; iii) estudos fotofísicos; iv) estudos teóricos.

Ultrafast transient absorption spectroscopy

The LCG has different array resolutions in order to identify Copy number variations (CNV) and detection of loss of heterozygosity. This diagnosis can be done on pre-natal and post-natal samples.

Cryopreservation in liquid nitrogen

LCG provides solutions in Genomics, Conventional and/or Molecular Cytogenetics and Molecular Biology for genetic studies of Developmental Disorders.

DNA extration from different biological samples.

These studies are carried out taking into account the alterations present in the index case, using the most appropriate techniques in the set of existing LCG techniques to characterize the alterations.

The study of the constitutional karyotype makes it possible to observe the entire genome, being able to identify both balanced and unbalanced structural chromosomal alterations, as well as alterations in the number of chromosomes and mosaics.

Infertility test by Cytogentics and Molecular Biology techniques.

EBV based lymphocyte immortalization

MS-MLPA can be used for the analysis of both methylation as well as copy number changes of syndromic related regions.

Molecular cytogenetics (FISH) allows a more detailed study of specific chromosomal regions, increasing the resolving power of conventional cytogenetics. The LCG provides an extensive library of FISH probes.

The rapid screening of the most common aneuploidies allows, in a short period of time (24h-72h), to evaluate the ploidy (number) of chromosomes 13, 18 and 21 and of the sex chromosomes X and Y.

MLPA can be used for the analysis of copy number changes in DNA of syndromic related regions

Array-CHG

Non invasive pre-natal test (NIPT)

The LCG performs a wide variety of exams in Prenatal. The fetal genome can be analyzed more widely, by molecular karyotype (aCGH) and constitutional karyotyope, More specific techniques like FISH, MLPA and MS-MLPA can also be performed whenever necessary.

Tissue culture of different tissues.

Annual 5-day course divided into theoretical, theoretical-practical sessions and 6 practical workshops, with the aim of addressing the evolution from cytogenetics to genomics, both in pre- and postnatal diagnosis and in research and forensic medicine

Dotar os meios humanos de entidades públicas e privadas dos conhecimentos e experiência necessária para implementar soluções sustentadas assentes em dados de Observação de Terra multimissão.

Apoio técnico (consultoria), co-desenvolvimento e distribuição de layers desenvolvidas in-house e por parceiros.

Programas de referência, atendendo às necessidades do mercado e métricas de performance internacionalmente aceites. Workshops temáticos e generalistas de capacitação em tópicos relevantes de Observação da Terra e análise de dados (e.g. machine-learning).

Ativação de comunidades de utilizadores em mercados tradicionais (e.g. floresta, gestão do território). Mapeamento de necessidades, caracterização de territórios e comunidades, desenho e implementação (ou apoio) de ações de ‘engagement’ dos utilizadores.

Molecular tumour profiling can predict an individual’s prognosis by interpreting the expression pattern of a panel of tumour-related genes.

This procedure allows to study DNA and RNA from cancer cells from a tumor that are circulating in the blood. May be used to help find cancer at an early stage and be used to help plan treatment.

Investigate the microbiota composition of complex environments. This application is based on the amplification of a specific region of the 16S rRNA gene for bacteria or the ITS (Internal Transcribed Spacer) region for fungi.

This application is commonly used to identify exon-level copy number variants (CNVs) not detected by conventional sequencing.

NGS panels are collections of genes that have been selected to identify a genetic cause of disease or to study regions of interest. It allows simultaneous sequencing of all the genes, in a faster and more cost-efffective way than conventional sequencing.

RNA-seq allows gene expression analysis across the whole transcriptome or specifically, reveal the presence and quantity of RNA of selected genes.

SNVs/SNPs identification. This application is also used to identify exon-level copy number variants (CNVs).

SNVs/SNPs identification.

Intervention areas

Earth Ecosystems Services - a Parternship in Rendering Services for Sedimentary Earth, Ecology and Surface waters, which integrates several technological platforms

What we do

Electrofisiologia

Imagem Animal

Aplicação Clinica: POC, Dor, Depressão

Aplicação Clinica: Tumores Neuroendocrinos

Aplicação Clinica: D. Parkinson

Aplicação Clinica: Epilepsia

Aplicação Clinica: Tumores Cerebrais

Aplicação Clinica: Infecção/Inflamação cerebral

Aplicação Clinica: Síndromes Parkinsónicos

Aplicação Clinica: Cardiologia

Diagnóstico em Carcinoma da Próstata

Aplicação Clinica: Oncologia

Aplicação Clinica: D. Parkinson

Estudo de corpo inteiro ou cardíaco

Aplicação Clinica: Metastização óssea

Aplicação Clinica: Carcinoma da Próstata

Aplicação Clinica: D. Alzheimer

Aplicação Clinica: D. Alzheimer

Exames de Diagnóstico e Investigação

Access to cluster Navigator for computing, storage, visualization and data backup. Support is available to all users through a ticketing system.

Organization of courses/workshops about advanced computing including: tools, libraries, resource management software, application software and accelerators.

HPC consulting, namely about the existing software environment, parallel computing and usage of containers for specific workflows

DNA/RNA target gene search with the Oncomine Precision Assay panel (50 genes)

DNA and RNA target gene search with the Oncomine Precision Assay panel (50 genes)

Isolation of nucleic acids from tissues and cells

Analysis of areas of interest; nuclear / cytological morphometry; evaluation of immunohistochemistry techniques

Immunohistochemistry (IHC) is a method of fiding antigens (e.g. proteins) in tissues by exploiting the principle of specific binding of antibodies to antigens in biological tissue.

Standard technique to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue

Technology that comprises the entire process from paraffin-embedded tissue sample to the result (nucleic acid isolation, amplification, real-time PCR detection and data analysis) in less than three hours

Scanning of slides for digital support

Scanning of slides for digital support

Molecular cytogenetic technique using fluorescent probes that bind only to certain regions of a nucleic acid sequence with a high degree of complementarity.

Cases from other services for a second opinion.

Section of paraffin block to prepare slides for further staining

Hematoxylin-eosin staining Test for Helycobacter Pylori

Determination of the compressive strength of hardened concrete specimens.

Assessment of the gross heat of combustion of products at constant volume in a bomb calorimeter.

Assessment of the ignitability of products by direct small-flame impingement using vertically oriented test specimens.

Assessment of the the non-combustibility performance, under specified conditions, of homogeneous products and substantial components of non-homogeneous products.

Assessment of the burning behaviour and spread of flame of horizontally mounted floorings exposed to a heat flux radiant gradient, when ignited with pilot flames.

Assessment of the burning behaviour and spread of flame of vertically mounted specimens (in a right angle) exposed to thermal attack by a single burning item (SBI).

Determination of the mechanical properties of metallic materials at room temperature.

Multiphoton microscopy on live or fixed samples, from different origins. Specialized technical training.

Confocal microscopy on live or fixed biological samples, from different origins. Specialized technical training.

Análise de expansões repetidas de nucleótidos (STR-PCR, TP-PCR)

Estudo de polimorfismos (PCR e RFLP)

Sequenciação de Nova Geração (NGS)

Determinação dos niveis de GRN por Imunoensaio enzimático (ELISA)

Sequenciação de Sanger

Data analytics; risk assessment; instrumentation; calibration; construction; geology; cartography; mitigation; remediation; Big data; others.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Determination of the proportion of biofuel in fuels.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Collection, preservation and transport of samples for analysis of radioactive parameters.

Collection, preservation and transport of samples for analysis of radioactive parameters.

Placement and/or collection of detectors for radon analysis.

Collection, preservation and transport of samples for analysis of radioactive parameters.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

At the end of the action, participants will be able to interpret the results and be aware of the most relevant aspects associated with their application in specific situations.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Data analytics; Big data; mitigation; remediation; construction; risk assessment; instrumentation; calibration; geology; cartography; others.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Ammonia Determination by Segmented Continuous Flow with Molecular Absorption Spectrometry

Determinação de

Determination and identification by species of the existing benthic community in sediment samples

Determination of Nitrates by Segmented Continuous Flow with Molecular Absorption Spectrometry

Determination of Nitrites by Segmented Continuous Flow with Molecular Absorption Spectrometry

Determination of Phosphate by Segmented Continuous Flow with Molecular Absorption Spectrometry

Determination of Silica by Segmented Continuous Flow with Molecular Absorption Spectrometry

Determination of Stable Isotopes by Mass Spectrometry (δ 13C (‰); δ 15N (‰); % C; % N)

Determination of Total Nitrogen by Segmented Continuous Flow with Molecular Absorption Spectrometry

Determination of Total Phosphorus by Segmented Continuous Flow with Molecular Absorption Spectrometry

Group of methodological assays to support Functional Genomics, an integrated analysis that aims to give a functional meaning to genetic mutations, particularly new alterations and/or of unknown significance, as a cause of disease.

Characterization of mRNA. Provides reliable and reproducible characterization of total RNA and mRNA. Objective measurement of RNA quality with RIN (RNA Integrity Number).

The laboratory provides functional mitochondrial testing for suspected bioenergetics disorders.

Allows identification of alterations in mitochondrial DNA, which may elucidate disease and bioenergetics dysfunction. Mitochondrial biology and pathophysiology is a complex field of research, which may help to answer a broad range of questions.

Sequencing of whole nuclear exome or individual genes (in familial/trio or individual), including bioinformatics analysis for pathogenicity evaluation. Confirmation of specific mutations.

Study of genes associated to pharmacogenomics. Study of biochemical biomarkers, like those associated to wellness.

Revestimento por película de cabono de amostras para análise/imagem por microscopia eletrónica de varrimento SEM

Aquisição de difratogramas de RX em materiais policristalinos (pó) à temperatura ambiente para identificação de fases, quantificação ou refinamento estrutural pelo método de Rietveld.

Measurement of electrical transport properties (resistivity, Hall effect, I(V) e dI/dV) in the temperature range 1.8 K-400 K and magnetic field 0-9 T.

Elemental quantitative analysis by mass spectrometry (ICP-MS) in diluted aqueous solutions

Imaging by Electron Scanning Microscopy (SEM)

Measurement of the thickness of thin films and coatings by X-ray fluorescence (XRF)

Metallic coating of samples for SEM imaging/ analysis by sputtering

Obtenção de imagem por microscopia de força atómica (AFM)

Aquisição de difratogramas de RX em materiais policristalinos (pó) à temperatura ambiente para identificação de fases, quantificação ou refinamento estrutural pelo método de Rietveld.

Medidas de magnetização em função da temperatura (1.8-400K) e campo magnético aplicado (0-9T) pela técnica VSM

Medição de propriedades de transporte térmico

Aquisição de difratogramas de RX em materiais policristalinos (pó) a temperatura variável (ambiente - 1450 ºC) para identificação de fases, quantificação ou refinamento estrutural pelo método de Rietveld.

Fornecimento de ácido nítrico ultra-puro, obtido por destilação em sub-eubição, para uso em preparação de amostras para análise por ICP-MS

Aquisição de perfis de refletometria de RX (XRR) para caracterização de filmes finos e revestimentos (espessura do filme, rugosidade, etc.)

Medição simultânea de variação de entalpia e de massa, em processos de aquecimento e arrefecimento, entre 20 e 1000 ºC para estudo e caracterização termodinâmica de transições de fase, decomposição térmica, etc.

Specific heat measurements as function of temperature and applied magnetic field.

Medidas de magnetização em função da temperatura (1.8-400K) e campo magnético aplicado (0-9T) pela técnica de magnetometria de torque

Obtenção de espectros de fluorescência de raios-X por excitação de feixe eletrónico (espectroscopia EDS)

Acquisition of 13C-NMR Spectrum

Acquisition of 1H-NMR spectrum.

Acquisition of 2D homonuclear NMR spectra

Acquisition of 2D heteronuclear NMR spectra

Acquisition of 1D NMR spectra for less common nuclei

1D 1H-NMR spectrum with absolute quantification of main species

Assignment of 1D and 2D NMR spectra and structure determination of small molecules from different origins

Serviços de consultoria especializada, nas áreas da saúde mental e do bem-estar, destinados a diferentes equipas, organizações e serviços.

Cursos de formação para a capacitação e desenvolvimento de diferentes profissionais na área da saúde mental.

Serviços de prevenção e tratamento da doença mental e de promoção do bem-estar emocional (Psicologia Clínica Cognitivo-Comportamental e Psiquiatria).

Serviços de supervisão clínica especializada para psicólogos.

Bacterial Fatty Acids identification and quantification by Gas Chromatography using the standard MIS Library Generation Software (Sherlock Microbial ID System).

Analysis of Polar Lipids from bacterial strains by Thin Layer Chromatography.

Analysis of Respiratory Quinones from bacterial strains by High Performance Liquid Chromatography.

Bacterial Strains from Public Collection: Active cultures | Freeze-dried strains

Bacteria detection on WLD selective medium | Bacteria identification | Hemocytometer yeast cell count | Beer specific gravity at 20 °C | pH | Total acidity | Color on SRM scale | Color on EBC scale | Beer bitterness | Total reducing sugars

Analysis of fatty acids (GC, MIDI) | Analysis of polar lipids (TLC) | Analysis of respiratory quinones (HPLC)

Whole genome analysis | Phylogenomic analysis

Public Deposit | Safe Deposit | Renew the Safe Deposit Service | Annual Maintenance Fee | Release of Strain

Genomic DNA Extraction | Sequencing of 16S rRNA gene | Phylogenetic analysis | Sequence Analysis of Genes | Mol% G+C Content Analysis of Genomic DNA | RAPD Fingerprinting for two Strains | Whole Genome Sequencing of one Prokaryotic strain

Isolation from a mixed culture plate | Preservation in −80 °C

Denaturing Gradient Gel Electrophoresis (DGGE) | Phospholipid Fatty Acid Analysis (PLFA) | Barcoding Sequencing

Multiproposes certificates (not related with describing of novel species) | Consultancy | Training and related services

Basic Phenotypic Characterization (based on API® strips) | BIOLOG Microplates (for extensive testing of oxidation of different carbon sources) | Morphological study (celular morphologyl, Gram, KOH, catalase, oxidase)

Plasmid design; Cloning; Vector design and engineering; Purification processes; Chromatography; Clean rooms; Instrumentation; Gene therapy; Cell models; Animal models; Disease models; Assays; Preclinical studies; Data analysis; Others.

Isolation of Extracellular Vesicles / Exosomes from diferent biological fluids

Production of highly pure AAVs (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, PhP.eB) using an optimized platform based on high performance liquid chromatography.

Production of highly pure lentiviral vectors using an optimized platform.

Production of modified exosomes - extracelular vesicles (EVs)

Measurement and Characterization of non-viral vectors (Extracellular vesicles / Exosomes) in various types of samples using a diferent set of assays.

Measurement of viral vector titer (AAV, LV, vesoxome) in various types of samples using a diferent set of assays.

Parâmetros a analisar: Microrganismos viáveis a 30ºC Listeria monocytogenes e Listeria spp. Salmonella spp. Enterobacteriaceae Bacillus cereus Staphylococcus aureus Campylobacter spp. Escherichia coli Clostridium perfringens Bolores e leveduras

Parâmetros a analisar Microrganismos viáveis a 30ºC Staphylococcus aureus Escherichia coli

Parâmetros a analisar: Microrganismos viáveis a 30ºC Listeria monocytogenes e Listeria spp. Enterobacteriaceae Staphylococcus aureus Escherichia coli Bolores e leveduras

Widefield Microscope with LED-based system (UV 385/30 nm, B 469/38 nm, G 555/30 nm, Y 590/27 nm, and R 631/33 nm) Scanning Confocal Microscope with 4 laser units (Diode 405 nm; Argon/2: 458, 488, 514 nm; DPSS: 561 nm and HeNe: 633 nm)

Observation and analysis of live or fixed samples.

Theoretical and practical training in optical and/or electron microscopy

The iLAB performs and provides the required materials and equipments necessary for sample processing for electron microscopy including fixation, dehydration, resin embedding and ultramicrotomy.