This course aims to give theoretical and practical fundaments of cell and tissue culture techniques. Therefore, we expect to enjoy this III Cell Culture and Tissue Training Course and take the best of it!

A single cell is the building block for life, reason why cell culture has become one of the major tools used in numerous fields of life sciences today. In its simplest form, cell culture is a complex process by which cells are dispersed under controlled conditions, in an artificial environment composed of nutrient solutions, in a suitable surface that allow cells to grow, with ideal conditions of temperature, humidity and gaseous atmosphere. In such a system, a researcher can precisely measure cells response to different alterations and analyze biological properties and processes that are not readily accessible at the level of the intact organism.Cell culture has become an essential tool for basic and translational research namely Medicine, Physiology, Biotechnology, Pharmaceutical or Cellular and Molecular Biology. As an excellent alternative for the use of animal models, this technique has emerged as an essential tool.

Theoretical course

  • Principles of cell culture
  • Advantages and constraints of cell culture
  • Problems and troubleshooting in cell cultures
  • Biosafety levels
  • Cell culture models
  • Primary cultures
  • Organotypic cell cultures
  • Flow cytometry
  • Microscopy
  • Statistics applied to cell culture assays

Practical course

  • Thawing and cryopreservation
  • Cell lines subculture
  • Primary cell cultures
  • Contamination detection
  • Viability, cytotoxicity and proliferation assays
  • Flow cytometry
  • Microscopy
  • Co-cultures
  • Database in cell culture assays

Registration Fees

Before 1st June - 200€

After 1st June - 230€

University of Coimbra Students - 180€

Full course attendance is limited to 25 participants

Only Theoretical Lectures (no limit of participants)

Post-graduated students - 50€

Undergraduated students - 30€

Program and Registration

Program (273 kB)
Registration (1 MB)

Travel Directions

Address:

Polo III - Health Sciences Campus- Azinhaga Santa Comba, Celas- 3000-548 Coimbra

Coordinates:

Latitude: 40.219412 ou 40º 13' 10'' N Longitude: -8.417008 ou 8º 25' 1'' W

TO REACH IBILI - COIMBRA, PORTUGAL

By air

Portugal has two main airports: Lisbon Airport and Oporto Airport. Lisbon Airport is 200 km away from Coimbra while Oporto Airport is 130 km away.

Transfer from Lisbon Airport to Coimbra

By car

If renting a car at the airport, you can drive to Coimbra by taking the highway A1 direction North (200Km – 2 hours). You must leave the highway at the exit “Coimbra Sul”. Leaving the highway, you will cross the river until you find the sign “Hospitais”. Follow this sign and you will reach

IBILI right by the Coimbra University Hospital (HUC).

By train

Take a taxi to the train station “ORIENTE” and there you may take Long Distance trains (expected price 258). In order to know the timetables, you may consult the website: www.cp.pt, and then you must choose “Timetables” – “Alfa Pendular and Intercidades” - “Lisboa/Porto-Braga-Guimarães”. You will see then the timetable of departure at “LISBOA-ORIENTE” and the timetable of arrival at “COIMBRA-B”. At COIMBRA-B train station, you may take a taxi to AIBILI

(expected price 58).

Transfer from Oporto Airport to Coimbra

By car

If renting a car at Oporto airport, you can drive to Coimbra by taking the highway A1 direction South, Lisbon (120Km – 1 hour). You must leave the highway at the exit “Coimbra Norte”. At IC2, you must leave at the exit on your right “Coimbra” and at the roundabout, you must always follow the sign “Hospitais”. You will then reach AIBILI right by the Coimbra University Hospital (HUC).

By train

Take a taxi to the train station “CAMPANHÔ and there you may take Long Distance trains (expected price 168). In order to know the timetables, you may consult the website: www.cp.pt, and then you must choose “Timetables” – “Alfa Pendular and Intercidades” - “Lisboa/Porto- Braga-Guimarães”. You will see then the timetable of departure at “PORTO CAMPANHA” and the timetable of arrival at “COIMBRA-B”. At COIMBRA-B train station, you may take a taxi to IBILI (expected price 58).

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