Portal

Extraction, hydrolysis, identification, and quantification of monosaccharides from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-RI).

Extraction, identification and quantification of pigments from microalgae and cyanobacteria by high-performance liquid chromatography (HPLC-DAD)

Extraction, identification and quantification of fatty acids from microalgae and cyanobacteria by gas chromatography (GC-FID).

Extraction and quantification of total lipid from microalgae and cyanobacteria by gravimetric methods; separation and quantification of neutral lipids, glycolipids and phospholipids by column chromatography

Extraction and quantification of total sugars of microalgae and cyanobacteria by gravimetric analysis and spectrophotometry

The Karl Fischer titration is a sensitive method to quantify the % water in various types of samples.

Mass percentage (% m/m) quantification of carbon (C), hydrogen (H), nitrogen (N), sulfur (S) and oxygen (O) present in a sample.

Quantitative and qualitative determination of ions (anions and cations).

Separation of the various components of a mixture, with the aim of identifying and quantifying these components.

Spectroscopic analysis methods consist of measuring the amount of radiation emitted or absorbed by molecules.

Study of the deformational behavior and the flow of matter subjected to stress, under certain thermodynamic conditions over a period of time. It includes properties such as: elasticity, viscosity and plasticity.

Determination of particle sizes (from 3 nm to 5000 μm). Aggregation, agglomeration and flocculation studies.

Determination of surface chemical and physical properties.

Study of the behavior of materials as a function of time or temperature, when heated, cooled or kept at a constant temperature.

i) viabilidade celular; ii) fototoxicidade; iii) "uptake"; e iv) mecanismo de morte celular

Infrared and Raman spectroscopic analysis applied to materials and food science, astrochemistry, arts and cultural heritage, and fundamental science

Cryopreservation in liquid nitrogen

LCG provides solutions in Genomics, Conventional and/or Molecular Cytogenetics and Molecular Biology for genetic studies of Developmental Disorders.

DNA extration from different biological samples.

These studies are carried out taking into account the alterations present in the index case, using the most appropriate techniques in the set of existing LCG techniques to characterize the alterations.

The study of the constitutional karyotype makes it possible to observe the entire genome, being able to identify both balanced and unbalanced structural chromosomal alterations, as well as alterations in the number of chromosomes and mosaics.

Infertility test by Cytogentics and Molecular Biology techniques.

EBV based lymphocyte immortalization

MS-MLPA can be used for the analysis of both methylation as well as copy number changes of syndromic related regions.

Molecular cytogenetics (FISH) allows a more detailed study of specific chromosomal regions, increasing the resolving power of conventional cytogenetics. The LCG provides an extensive library of FISH probes.

The rapid screening of the most common aneuploidies allows, in a short period of time (24h-72h), to evaluate the ploidy (number) of chromosomes 13, 18 and 21 and of the sex chromosomes X and Y.

MLPA can be used for the analysis of copy number changes in DNA of syndromic related regions

Array-CHG

Non invasive pre-natal test (NIPT)

The LCG performs a wide variety of exams in Prenatal. The fetal genome can be analyzed more widely, by molecular karyotype (aCGH) and constitutional karyotyope, More specific techniques like FISH, MLPA and MS-MLPA can also be performed whenever necessary.

Tissue culture of different tissues.

Molecular tumour profiling can predict an individual’s prognosis by interpreting the expression pattern of a panel of tumour-related genes.

This procedure allows to study DNA and RNA from cancer cells from a tumor that are circulating in the blood. May be used to help find cancer at an early stage and be used to help plan treatment.

Investigate the microbiota composition of complex environments. This application is based on the amplification of a specific region of the 16S rRNA gene for bacteria or the ITS (Internal Transcribed Spacer) region for fungi.

This application is commonly used to identify exon-level copy number variants (CNVs) not detected by conventional sequencing.

NGS panels are collections of genes that have been selected to identify a genetic cause of disease or to study regions of interest. It allows simultaneous sequencing of all the genes, in a faster and more cost-efffective way than conventional sequencing.

RNA-seq allows gene expression analysis across the whole transcriptome or specifically, reveal the presence and quantity of RNA of selected genes.

SNVs/SNPs identification. This application is also used to identify exon-level copy number variants (CNVs).

SNVs/SNPs identification.

DNA/RNA target gene search with the Oncomine Precision Assay panel (50 genes)

DNA and RNA target gene search with the Oncomine Precision Assay panel (50 genes)

Isolation of nucleic acids from tissues and cells

Analysis of areas of interest; nuclear / cytological morphometry; evaluation of immunohistochemistry techniques

Immunohistochemistry (IHC) is a method of fiding antigens (e.g. proteins) in tissues by exploiting the principle of specific binding of antibodies to antigens in biological tissue.

Standard technique to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue

Technology that comprises the entire process from paraffin-embedded tissue sample to the result (nucleic acid isolation, amplification, real-time PCR detection and data analysis) in less than three hours

Scanning of slides for digital support

Molecular cytogenetic technique using fluorescent probes that bind only to certain regions of a nucleic acid sequence with a high degree of complementarity.

Section of paraffin block to prepare slides for further staining

Hematoxylin-eosin staining Test for Helycobacter Pylori

Multiphoton microscopy on live or fixed samples, from different origins. Specialized technical training.

Confocal microscopy on live or fixed biological samples, from different origins. Specialized technical training.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Determination of the proportion of biofuel in fuels.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Collection, preservation and transport of samples for analysis of radioactive parameters.

Collection, preservation and transport of samples for analysis of radioactive parameters.

Placement and/or collection of detectors for radon analysis.

Collection, preservation and transport of samples for analysis of radioactive parameters.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Measurement of naturally-occurring radionuclides of natural, human-made or both origins, that may pose a risk to human health.

Characterization of mRNA. Provides reliable and reproducible characterization of total RNA and mRNA. Objective measurement of RNA quality with RIN (RNA Integrity Number).

Allows identification of alterations in mitochondrial DNA, which may elucidate disease and bioenergetics dysfunction. Mitochondrial biology and pathophysiology is a complex field of research, which may help to answer a broad range of questions.

Sequencing of whole nuclear exome or individual genes (in familial/trio or individual), including bioinformatics analysis for pathogenicity evaluation. Confirmation of specific mutations.

Study of genes associated to pharmacogenomics. Study of biochemical biomarkers, like those associated to wellness.

Elemental quantitative analysis by mass spectrometry (ICP-MS) in diluted aqueous solutions

Imaging by Electron Scanning Microscopy (SEM)

Isolation of Extracellular Vesicles / Exosomes from diferent biological fluids

Production of highly pure AAVs (AAV1, AAV2, AAV5, AAV6, AAV8, AAV9, PhP.eB) using an optimized platform based on high performance liquid chromatography.

Production of highly pure lentiviral vectors using an optimized platform.

Production of modified exosomes - extracelular vesicles (EVs)

Parâmetros a analisar: Microrganismos viáveis a 30ºC Listeria monocytogenes e Listeria spp. Salmonella spp. Enterobacteriaceae Bacillus cereus Staphylococcus aureus Campylobacter spp. Escherichia coli Clostridium perfringens Bolores e leveduras

Parâmetros a analisar Microrganismos viáveis a 30ºC Staphylococcus aureus Escherichia coli

Parâmetros a analisar: Microrganismos viáveis a 30ºC Listeria monocytogenes e Listeria spp. Enterobacteriaceae Staphylococcus aureus Escherichia coli Bolores e leveduras

Observation and analysis of live or fixed samples.

The iLAB performs and provides the required materials and equipments necessary for sample processing for electron microscopy including fixation, dehydration, resin embedding and ultramicrotomy.